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Trypticase™ Phytone™ Glucose Medium

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 15.0g

Pancreatic digest of casein.......................................................... 10.0g

Agar .............................................................................................. 8.0g

Papaic digest of soybean meal ...................................................... 5.0g

Yeast extract.................................................................................. 2.5g

K2HPO4......................................................................................... 2.0g

L-Cysteine·HCl·H2O...................................................................... 0.5g

MgCl2............................................................................................ 0.5g

ZnSO4·7H2O ............................................................................... 0.25g

FeCl3 ..........................................................................................1.0mg

Preparation of Medium: Add ZnSO4 to approximately 100.0mL

of distilled/deionized water and dissolve. Add remaining components

and bring volume to 1.0L with distilled/deionized water. Mix thor

oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bifidobacterium species.