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Trypticase™ Soy Agar, Modified (ATCC Medium 1386)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar, Modified

(ATCC Medium 1386)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 18.0g

Pancreatic digest of casein.......................................................... 17.0g

NaCl.............................................................................................. 5.0g

Papaic digest of soybean meal...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

Yeast extract.................................................................................. 0.4g

NH4OH, concentrated............................................................0.035mL

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except NH4OH, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add NH4OH. Mix thoroughly.

Adjust pH to 7.5 if necessary. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation of ATCC strain 31205.