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Trypticase™ Soy Agar, Modified (ATCC Medium 1481)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar, Modified

(ATCC Medium 1481)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Papaic digest of soybean meal...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

L-Glutamine .............................................................................10.0mL

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except glutamine, to

distilled/deionized water and bring volume to 990.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile

glutamine. Mix thoroughly. Adjust pH to 6.5. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: Used as a base that is supplemented. For the cultivation of fastid

ious microorganisms. When supplemented with sheep blood, this

medium is useful for the observation of hemolytic reactions of a variety

of bacteria.