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Trypticase™ Soy Agar, Modified (TSA II™)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar, Modified

(TSA II™)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 14.5g

Agar ............................................................................................ 14.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 5.0g

Growth factors (BBL)................................................................... 1.5g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile

Petri dishes or leave in tubes. For blood plates, 50.0–100.0mL of sterile

defibrinated sheep blood may be added to sterile medium that has been

melted and cooled to 45°–50°C.

Use: Used as a base that is supplemented. For the cultivation of fastid

ious microorganisms. When supplemented with sheep blood, this

medium is useful for the observation of hemolytic reactions of a variety

of bacteria. It may be used to perform the CAMP test for the presump

tive identification of group B streptococci (Streptococcus agalactiae).