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Trypticase™ Soy Agar, Modified with Horse Serum

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]
Trypticase™ Soy Agar, Modified with Horse Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g
Agar ............................................................................................ 15.0g
NaCl .............................................................................................. 5.0g
Yeast extract.................................................................................. 4.0g
Papaic digest of soybean meal ...................................................... 3.0g
K2HPO4......................................................................................... 2.5g
Horse serum ...........................................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se
rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of Simonsiella species,
Alysiella species, and Moraxella species.
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