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Trypticase™ Soy Agar with Sheep Blood (Tryptic Soy Blood Agar) (TSA Blood Agar)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar with Sheep Blood

(Tryptic Soy Blood Agar)

(TSA Blood Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 15.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 5.0g

Sheep blood, defibrinated ........................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep

blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL vol

umes or distribute into sterile tubes.

Use: For the cultivation of a wide variety of fastidious microorganisms.

For the observation of hemolytic reactions of a variety of bacteria. May be

used to perform the CAMP test for the presumptive identification of group

B streptococci (Streptococcus agalactiae).