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Trypticase™ Soy Agar with Sheep Blood, Formate, and Fumarate

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar

with Sheep Blood, Formate, and Fumarate

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 14.5g

Agar ............................................................................................ 14.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 5.0g

Sucrose.......................................................................................... 2.0g

Growth factors .............................................................................. 1.5g

Sheep blood, defibrinated ........................................................50.0mL

Formate-fumarate solution.......................................................13.0mL

pH 7.3 ± 0.2 at 25°C

Source: Growth Factors are available as a premixed powder from BD

Diagnostic Systems.

Formate-Fumarate Solution:

Composition per 100.0mL:

Sodium formate ............................................................................ 6.0g

Fumaric acid ................................................................................. 6.0g

Preparation of Formate-Fumarate Solution: Add components

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Adjust pH to 7.0. Filter sterilize.

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thor

oughly. Pour into sterile Petri dishes. Prior to inoculation, aseptically

spread 0.2mL of sterile formate-fumarate solution on each plate.

Use: For the isolation of Streptococcus pneumoniae from a variety of

clinical specimens.