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Trypticase™ Soy Agar with Sheep Blood and Gentamicin (TSA II™ with Sheep Blood and Gentamicin)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar with

Sheep Blood and Gentamicin

(TSA II™ with Sheep Blood and Gentamicin)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 14.5g

Agar ............................................................................................ 14.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 5.0g

Growth factors .............................................................................. 1.5g

Sheep blood, defibrinated ........................................................50.0mL

Gentamicin solution.................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Gentamicin Solution:

Composition per 10.0mL:

Gentamicin.................................................................................2.5mg

Preparation of Gentamicin Solution: Add gentamicin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except sheep blood

and gentamicin solution, to distilled/deionized water and bring vol

ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile sheep blood and sterile gentamicin solution.

Mix thoroughly. Pour into sterile Petri dishes or distribute into ster

ile tubes.

Use: For the isolation of Streptococcus pneumoniae from a variety of

clinical specimens.