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Trypticase™ Soy Agar with Sheep Blood and Streptomycin (ATCC Medium 1810)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar with

Sheep Blood and Streptomycin

(ATCC Medium 1810)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 14.5g

Agar ............................................................................................ 14.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 5.0g

Growth factors .............................................................................. 1.5g

Sheep blood, defibrinated ........................................................50.0mL

Streptomycin solution ..............................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Source: Growth factors are available as a premixed powder from BD

Diagnostic Systems.

Streptomycin Solution:

Composition per 10.0mL:

Streptomycin ................................................................................. 0.5g

Preparation of Streptomycin Solution: Add streptomycin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except sheep blood

and streptomycin solution, to distilled/deionized water and bring

volume to 940.0mL. Mix thoroughly. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add sterile sheep blood and sterile streptomycin

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation of Streptococcus gordonii.