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Tryptone Bile Glucuronide Agar, Harlequin (Harlequin TBGA)

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Bile Glucuronide Agar, Harlequin

(Harlequin TBGA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone...................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Bile salts No. 3.............................................................................. 1.5g

X-glucuronide ........................................................................... 0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from lab m.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Allow to soak for 10

min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Pour into sterile Petri dishes.

Use: For the simple enumeration of E. coli without the need for mem

branes or pre-incubation The medium has been modified by the addi

tion of a chromogenic substrate to detect the β-glucuronidase, which is

highly specific for E. coli. The advantage of the chromogenic substrate

is that it requires no UV lamp to visualize the reaction, and it is con

centrated within the colony, facilitating easier enumeration in the pres

ence of other organisms, or when large numbers are present on the

plate.