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Tryptone Bile X-glucuronide Agar, Chromocult (Chromocult® TBX ) (ChromocultTryptone Bile X-glucuronide Agar)

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Bile X-glucuronide Agar, Chromocult

(Chromocult® TBX )

(ChromocultTryptone Bile

X-glucuronide Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ...................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Bile salts No. 3 ............................................................................. 1.5g

X-β-D-glucuronide ................................................................... 0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes.

Use: For the differentiation of E. coli from other coliforms. The pres

ence of the enzyme β-D-glucuronidase differentiates most E. coli spp.

from other coliforms. E. coli absorbs the chromogenic substrate 5-

bromo-4-chloro-3-indolyl-β-D-glucuronide (X-β-D-glucuronide). The

enzyme β-glucuronidase splits the bond between the chromophore 5-

bromo-4-chloro-3-indolyle and the β-D-glucuronide. E. coli colonies are

colored blue-green. Growth of accompanying Gram-positive flora is

largely inhibited by the use of bile salts. The prepared medium is clear

and yellowish.