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Tryptone Broth

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Glucose ......................................................................................... 5.0g

K2HPO4....................................................................................... 1.25g

Yeast extract.................................................................................. 1.0g

Bromcresol Purple solution .......................................................2.0mL

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ........................................................................ 2.0g

Ethanol.....................................................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol

Purple to 10.0mL of ethanol. Mix thoroughly. Bring volume to

100.0mL with distilled/deionized water.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into screw

capped tubes in 10.0mL volumes. Autoclave for 20 min at 15 psi pres

sure–121°C.

Use: For the cultivation of Salmonella species from foods.