培养基配方

您现在的位置：网站首页 >> 培养基配方

Tryptone Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Agar .............................................................................................. 2.0g

Yeast extract.................................................................................. 1.0g

Bromcresol Purple ...................................................................... 0.04g

Carbohydrate solution............................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate............................................................................... 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 100.0mL. Glucose or

mannitol may be used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except carbohydrate

solution, to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling.

Distribute into tubes in 13.5mL volumes. Autoclave for 20 min at 10

psi pressure–115°C. Cool to 45°–50°C. Aseptically add 1.5mL of car

bohydrate solution to each tube. Mix thoroughly. Solidify agar quickly

by placing tubes in ice water.

Use: For the cultivation and differentiation of Staphylococcus aureus

based on glucose and mannitol fermentation. Bacteria that ferment the

added carbohydrate turn the medium yellow.