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Tryptone Yeast Extract Agar 1

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Pancreatic digest of casein.......................................................... 10.0g

Yeast extract.................................................................................. 5.0g

K2HPO4 ........................................................................................ 4.4g

Glucose ......................................................................................... 2.0g

NaCl.............................................................................................. 2.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of a wide variety of het

erotrophic bacteria.