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Tryptone Yeast Extract Salt Medium

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL

Solution B ..............................................................................500.0mL

pH 6.8 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

NaCl.......................................................................................... 125.0g

MgCl2·6H2O ............................................................................... 50.0g

K2SO4 ........................................................................................... 5.0g

CaCl2·6H2O .................................................................................. 0.2g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.8.

Autoclave for 15 min at 15 psi pressure–121°C.

Solution B:

Composition per 500.0mL:

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 5.0g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.8.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 500.0mL of solu

tion A with 500.0mL of solution B. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation of Haloferax volcanii.