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Tryptophan Assay Medium

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 40.0g

Sodium acetate............................................................................ 20.0g

Casamino acids ........................................................................... 12.0g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.4g

L-Cystine ....................................................................................... 0.2g

Adenine sulfate ........................................................................... 0.02g

FeSO4·7H2O................................................................................ 0.02g

Guanine·HCl ............................................................................... 0.02g

MnSO4·7H2O.............................................................................. 0.02g

NaCl............................................................................................ 0.02g

Uracil .......................................................................................... 0.02g

Pyridoxine·HCl .......................................................................... 0.4mg

Riboflavin .................................................................................. 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Calcium pantothenate ................................................................ 0.2mg

Niacin......................................................................................... 0.2mg

Thiamine·HCl ............................................................................0.2mg

Biotin ..........................................................................................0.8μg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

in 5.0mL volumes. Add standard solutions and test solutions to each

tube. Bring volume of each tube to 10.0mL. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For the assay of tryptophan using Lactobacillus plantarum as an

indicator organism.