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TSC Agar, Fluorocult (Fluorocult TSC Agar) (Fluorocult Tryptose Sulfite Cycloserine Agar) (Tryptose Sulfite Cycloserine Agar, Fluorocult)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TSC Agar, Fluorocult

(Fluorocult TSC Agar)

(Fluorocult Tryptose Sulfite Cycloserine Agar)

(Tryptose Sulfite Cycloserine Agar, Fluorocult)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Tryptose ...................................................................................... 15.0g

Peptone from soymeal .................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Na2S2O5 ........................................................................................ 1.0g

Ammonium ferric citrate .............................................................. 1.0g

D-Cycloserine................................................................................ 0.2g

4-Methylumbelliferyl-phosphate disodium salt.......................50.0mg

Source: This medium is available from Merck.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes.

Use: For the isolation and enumeration of the vegetative and spore

forms of Clostridium perfringens in foodstuffs. The culture medium

complies with the recommendations of the International Organization

for Standardization (ISO) (1978) and the DIN Norm 10165 for the

examination of meat and meat products. It also conforms with the

APHA recommendations for the examination of foods (1992). D

Cycloserine inhibits the accompanying bacterial flora and causes the

colonies which develop to remain smaller. 4-Methylumbelliferyl-phos

phate (MUP) is a fluorogenic substrate for the alkaline and acid phos

phatase. The acid phosphatase is a highly specific indicator for C. per

fringens. The acid phosphatase splits the fluorogenic substrate MUP

forming 4-methylumbelliferone which can be identified as fluores

cence in long-wave UV light. Thus a strong suggestion for the presence

of C. perfringens can be obtained.