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TSN Agar (Trypticase™ Sulfite Neomycin Agar)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TSN Agar

(Trypticase™ Sulfite Neomycin Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 13.5g

Yeast extract................................................................................ 10.0g

Na2SO3.......................................................................................... 1.0g

Ferric citrate.................................................................................. 0.5g

Neomycin sulfate........................................................................ 0.05g

Polymyxin sulfate ....................................................................... 0.02g

Buffered thioglycolate solution ...............................................50.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Buffered Thioglycolate Solution:

Composition per 50.0mL:

Buffer solution .........................................................................35.0mL

Sodium thioglycolate solution .................................................15.0mL

Preparation of Buffered Thioglycolate Solution: Combine

components. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C.

Buffer Solution:

Composition per 100.0mL:

Na2CO3 ....................................................................................... 28.0g

K2HPO4......................................................................................... 5.7g

Preparation of Buffer Solution: Add components to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly.

Thioglycolate Solution:

Composition per 100.0mL:

Sodium thioglycolate.................................................................. 13.3g

Preparation of Thioglycolate Solution: Add sodium thioglyco

late to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly.

Preparation of Medium: Add components, except buffered thio

glycolate solution, to distilled/deionized water and bring volume to

950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 12 min at 13 psi pressure–118°C. Do not overheat. Cool to 45°–

50°C. Aseptically add buffered thioglycolate solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Clostridium perfringens.