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TTYSH Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TTYSH Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Tryptose ...................................................................................... 10.0g

Yeast extract................................................................................ 10.0g

Glucose ......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

L-Cysteine·HCl ............................................................................. 1.0g

K2HPO4......................................................................................... 0.8g

KH2PO4......................................................................................... 0.8g

Ascorbic acid ................................................................................ 0.2g

Bovine serum, heat inactivated................................................50.0mL

Hemin solution.........................................................................20.0mL

Hemin Solution:

Composition per 50.0mL:

Hemin.......................................................................................50.0mg

Preparation of Hemin Solution: Add 50.0mg of hemin to dis

tilled/deionized water and bring volume 50.0mL. Adjust the pH to 10.5

with 1N NaOH. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except tryptose, hemin

solution, and bovine serum, to distilled/deionized water and bring vol

ume to 600.0mL. Mix thoroughly. Add tryptose. Mix thoroughly. Gen

tly heat and bring to boiling. Cool to 25°C. Autoclave for 15 min at 15

psi pressure–121°C. Aseptically add 50.0mL of sterile, heat-inactivat

ed bovine serum and 20.0mL of sterile hemin solution. Mix thorough

ly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Crithidia fasciculata.