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TweenTM 80A Agar

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TweenTM 80A Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 3.0g

MgSO4·7H2O ................................................................................ 2.0g

Tween™ 80 solution ................................................................50.0mL

pH 7.2 ± 0.2 at 25°C

Tween™ 80 Solution:

Composition per 50.0mL:

Tween™ 80................................................................................. 10.0g

Preparation of Tween™ 80 Solution: Add Tween™ 80 to dis

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 so

lution, to distilled/deionized water and bring volume to 950.0mL. Gen

tly heat and bring to boiling. Adjust pH to 7.2. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of

sterile Tween™ 80 solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation of Agitococcus lubricus.