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Tween™ 80 Agar (DSMZ Medium 884)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

Tween™ 80 Agar

(DSMZ Medium 884)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................900.0mL

Solution B ..............................................................................100.0mL

Solution A:

Composition per 900.0mL:

Agar ............................................................................................ 15.0g

Peptone........................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

CaCl2·2H2O................................................................................... 0.1g

pH 7.1 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C.

Solution B:

Composition per 100.0mL:

Tween™ 80................................................................................. 10.0g

Preparation of Solution B: Add Tween™ 80 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 900.0mL sterile so

lution A and 100.0mL sterile solution B. Mix thoroughly. Pour into

sterile Petri dishes.

Use: For the cultivation of unclassified bacterium DSM 13023.