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TYE HES Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TYE HES Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 950.0mL:

NaCl............................................................................................ 49.7g

MgSO4·7H2O.............................................................................. 49.3g

Noble agar................................................................................... 10.0g

Yeast extract.................................................................................. 0.5g

Pancreatic digest of casein............................................................ 0.5g

CaCl2·2H2O solution................................................................50.0mL

pH 7.2 ± 0.2 at 25°C

CaCl2·2H2O Solution:

Composition per 100.0mL:

CaCl2·2H2O .................................................................................. 0.3g

Preparation of CaCl2·2H2O Solution: Add the CaCl2·2H2O to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, except CaCl2·2H2O so

lution, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of

sterile CaCl2·2H2O solution. Mix thoroughly. Adjust pH to 7.2. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Planococcus species.