编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

TYEG Medium (Trypticase™ Yeast Extract Glucose Medium)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TYEG Medium

(Trypticase™ Yeast Extract Glucose Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

NaCl.......................................................................................... 100.0g

Pancreatic digest of casein.......................................................... 10.0g

Na2HPO4·7H2O............................................................................. 2.1g

NH4Cl ........................................................................................... 1.0g

KH2PO4......................................................................................... 0.3g

MgCl2·6H2O ................................................................................. 0.2g

Glucose solution ......................................................................50.0mL

Na2S·7H2O solution.................................................................25.0mL

Trace minerals solution II........................................................10.0mL

Wolfe’s vitamin solution..........................................................10.0mL

Yeast extract solution.................................................................5.0mL

Resazurin (0.2% solution)..........................................................1.0mL

FeSO4·9H2O (2.5% solution).....................................................25.0μl

pH 7.3 ± 0.1 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose .................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce.

Na2S·7H2O Solution:

Composition per 100.0mL:

Na2S·7H2O.................................................................................... 2.5g

Preparation of Na2S·7H2O Solution: Add Na2S·7H2O to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Use freshly prepared

solution.

Trace Minerals Solution II:

Composition per liter:

Nitrilotriacetic acid ..................................................................... 12.8g

CoCl2·6H2O ................................................................................ 0.17g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O................................................................................. 0.1g

MnCl2·4H2O.................................................................................. 0.1g

NaCl .............................................................................................. 0.1g

ZnCl2............................................................................................. 0.1g

NiSO4·6H2O.............................................................................. 0.026g

CuCl2·2H2O ................................................................................ 0.02g

Na2SeO3 .................................................................................... 0.017g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Trace Minerals Solution II: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Filter through Whatman filter paper. Store under N2.

Wolfe’s Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................10.0mg

Thiamine·HCl ............................................................................5.0mg

Riboflavin ..................................................................................5.0mg

Nicotinic acid.............................................................................5.0mg

Calcium pantothenate ................................................................5.0mg

p-Aminobenzoic acid.................................................................5.0mg

Thioctic acid ..............................................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid....................................................................................2.0mg

Cyanocobalamin .....................................................................100.0μg

Preparation of Wolfe’s Vitamin Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Filter sterilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce.

Yeast Extract Solution:

Composition per 100.0mL:

Yeast extract................................................................................ 10.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Fil

ter sterilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce.

Preparation of Medium: Add components—except glucose solu

tion, yeast extract solution, and Wolfe’s vitamin solution—to distilled/de

ionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH

to 7.3. Gently heat and bring to boiling under 90% N2 + 10% CO2. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

and anaerobically add 50.0mL of sterile glucose solution, 10.0mL of ster

ile Wolfe’s vitamin solution, and 5.0mL of sterile yeast extract solution.

Aseptically and anaerobically distribute into sterile tubes in 5.0mL vol

umes. Immediately prior to inoculation, aseptically add 0.125mL of sterile

Na2S·9H2O solution per tube.

Use: For the cultivation and maintenenace of Halobacteroides aceto

ethylicus.

在线客服

TYEG Medium (Trypticase™ Yeast Extract Glucose Medium)