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TYG Medium
[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]
TYG Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Yeast extract.................................................................................. 5.0g
K2HPO4 ........................................................................................ 3.5g
Tryptone........................................................................................ 1.0g
L-Cysteinium chloride·H2O .......................................................... 0.5g
Na2SO4.......................................................................................... 0.2g
Biotin ....................................................................................... 10.0mg
p-Aminobenzoic acid............................................................... 10.0mg
Sugar solution ............................................................................5.0mL
Resazurin solution .....................................................................4.0mL
Trace elements solution .............................................................1.0mL
Resazurin Solution:
Composition per 100.0mL:
Resazurin ................................................................................. 25.0mg
Preparation of Resazurin Solution: Add resazurin to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per 100.0mL:
FeCl3·6H2O................................................................................... 2.7g
MgSO4 .......................................................................................... 1.2g
NaMoO4·2H2O ........................................................................... 0.24g
MnSO4·7H2O.............................................................................. 0.17g
CaCl2·2H2O ................................................................................ 0.15g
ZnSO4·7H2O............................................................................ 29.0mg
CuSO4·5H2O............................................................................ 25.0mg
CoCl2·6H2O............................................................................. 24.0mg
H2SO4 ........................................................................................2.8mL
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough
ly.
Sugar Solution:
Composition per 100.0mL:
Glucose ......................................................................................... 1.0g
Preparation of Sugar Solution: Add glucose to distilled/deion
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, except L-cysteinium
chloride, biotin, p-aminobenzoic acid, and sugar solution, to distilled/
deionized water and bring volume to 995.0mL. Mix thoroughly. Adjust
pH to 7.0. Gently heat but do not boil. Cook for 5–10 min so that color
first turns red and then turns yellow. Cool on ice. Add L-cysteinium
chloride, biotin, and p-aminobenzoic acid. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically add 5.0 mL of sterile
sugar solution. Mix thoroughly.
Use: For the cultivation of Clostridium species.