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TYG Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TYG Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Glucose ......................................................................................... 5.0g

KH2PO4......................................................................................... 4.0g

Sodium thioglycolate .................................................................... 0.5g

Yeast extract.................................................................................. 0.5g

MgSO4·7H2O ................................................................................ 0.2g

FeSO4·7H2O...............................................................................5.0mg

MnSO4·4H2O .............................................................................5.0mg

NH4MoO4 ..................................................................................5.0mg

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 with

NaOH. Distribute into tubes or flasks. Autoclave for 10 min at 11 psi

pressure–116°C.

Use: For the cultivation of Sprolactobacillus cellulosolvens.