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TYM Basal Medium, Modified 2

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Yeast extract................................................................................ 10.0g

Maltose ......................................................................................... 5.0g

L-Cysteine·HCl ............................................................................. 1.0g

K2HPO4......................................................................................... 0.8g

KH2PO4......................................................................................... 0.8g

Agar, noble.................................................................................... 0.5g

L-Ascorbic acid............................................................................. 0.2g

Lamb serum, heat inactivated................................................300.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar and lamb

serum, to distilled/deionized water and bring volume to 700.0mL. Mix

thoroughly. Adjust to pH 7.0 with NaOH. Add the agar. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C. Aseptically add 300.0mL of sterile, heat-in

activated lamb serum. Mix thoroughly. Aseptically distribute into sterile

screw-capped tubes. Use soon after preparation.

Use: For the cultivation of Trichomonas vaginalis and Hypotrichomo

nas species.