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TYM Basal Medium, Modified 3

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Yeast extract................................................................................ 10.0g

Maltose ......................................................................................... 5.0g

L-Cysteine·HCl ............................................................................. 1.0g

K2HPO4......................................................................................... 0.8g

KH2PO4......................................................................................... 0.8g

Agar, noble.................................................................................... 0.5g

L-Ascorbic acid............................................................................. 0.2g

Horse serum, heat inactivated................................................300.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar and horse se

rum, to distilled/deionized water and bring volume to 700.0mL. Mix thor

oughly. Adjust pH to 7.0. Add the agar. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 300.0mL of heat-inactivated horse serum. Mix

thoroughly. Aseptically distribute into screw-capped tubes.

Use: For the cultivation of Pentatrichomonas hominis, Proteromonas

lacertae, and Tritrichomonas foetus.