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Tyrosine Agar

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

Tyrosine Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................900.0mL

Solution 2...............................................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Solution 1:

Composition per 900.0mL:

Agar ............................................................................................ 15.0g

Pancreatic digest of gelatin........................................................... 5.0g

Beef extract................................................................................... 3.0g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling.

Solution 2:

Composition per 100.0mL:

Tyrosine ........................................................................................ 5.0g

Preparation of Solution 2: Add tyrosine to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Gently heat and

bring to boiling.

Preparation of Medium: Combine solutions 1 and 2. Mix thor

oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of aerobic Actinomycete species. Clearing

around a colony indicates utilization of tyrosine. Streptomyces and Actino

madura species utilize tyrosine. Nocardia asteroides, Nocardia caviae,

and Mycobacterium fortuitum do not utilize tyrosine.