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Tyrosine Agar (International Streptomyces Project Medium 7) (ISP Medium 7) (ATCC Medium 1776)

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

Tyrosine Agar

(International Streptomyces Project Medium 7)

(ISP Medium 7)

(ATCC Medium 1776)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Glycerol ...................................................................................... 15.0g

L-Tyrosine ..................................................................................... 0.5g

L-Asparagine ................................................................................. 1.0g

K2HPO4......................................................................................... 0.5g

MgSO4·7H2O ................................................................................ 0.5g

NaCl .............................................................................................. 0.5g

FeSO4·7H2O................................................................................ 0.01g

Trace elements solution HO-LE ................................................1.0mL

pH 7.3 ± 0.1 at 25°C

Trace Elements Solution HO-LE:

Composition per liter:

H3BO3 ......................................................................................... 2.85g

MnCl2·4H2O.................................................................................. 1.8g

Sodium tartrate............................................................................ 1.77g

FeSO4·7H2O................................................................................ 1.36g

CoCl2·6H2O ................................................................................ 0.04g

CuCl2·2H2O............................................................................... 0.027g

Na2MoO4·2H2O ........................................................................ 0.025g

ZnCl2........................................................................................... 0.02g

Preparation of Trace Elements Solution HO-LE: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of Streptoalloteichus species.

For the differentiation of Streptomyces species based on melanine produc

tion.