培养基配方

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TZC Selective Medium

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 17.0g

Peptone ......................................................................................... 1.0g

Glucose ......................................................................................... 0.5g

Pancreatic digest of casein............................................................ 0.1g

2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL

2,3,5-Triphenyltetrazolium·HCl Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium·HCl ................................................. 0.05g

Preparation of 2,3,5-Triphenyltetrazolium·HCl Solution: Add

2,3,5-triphenyltetrazolium·HCl to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C.

Preparation of Medium: Add components, except 2,3,5-

triphenyltetrazolium·HCl solution, to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 10.0mL of sterile 2,3,5-triphenyltetrazol

ium·HCl solution. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the isolation, cultivation, and differentiation of Pseudomonas

solanacearum. The virulent, wild-type strains appear as irregular to

round white colonies with a pink center. Avirulent mutants, which

readily occur in nature, appear as round, deep red colonies with a nar

row blue border.