编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

U Agar Plates (Ureaplasma Agar Plates) (MES Agar)

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

U Agar Plates

(Ureaplasma Agar Plates)

(MES Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.2mL:

Base agar..................................................................................65.0mL

Horse serum .............................................................................20.0mL

Yeast dialysate..........................................................................10.0mL

MES (2-N-morpholinoethane

sulfonic acid) buffer solution...............................................3.0mL

Penicillin solution ......................................................................2.0mL

Urea solution..............................................................................0.2mL

pH 5.5 ± 0.2 at 25°C

Base Agar:

Composition per liter:

Papaic digest of soybean meal .................................................... 20.0g

Agarose ....................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Phenol Red (2% solution) ..........................................................1.0mL

Preparation of Base Agar: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C.

Yeast Dialysate:

Composition per 10.0mL:

Yeast, active dried..................................................................... 450.0g

Preparation of Yeast Dialysate: Add active, dried yeast to dis

tilled/deionized water and bring volume to 1250.0mL. Gently heat and

bring to 40°C. Autoclave for 15 min at 15 psi pressure–121°C. Put into

dialysis tubing. Dialyze against 1.0L of distilled/deionized water for 2

days at 4°C. Discard tubing and its contents. Autoclave dialysate for 15

min at 15 psi pressure–121°C. Store at −20°C.

MES Buffer Solution:

Composition per 100.0mL:

MES (2-N-morpholinoethane

sulfonic acid) buffer ........................................................... 19.52g

Preparation of MES Buffer Solution: Add MES buffer to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Adjust pH to 5.5. Filter sterilize.

Penicillin Solution:

Composition per 10.0mL:

Penicillin .............................................................................. 100,000U

Preparation of Penicillin Solution: Add penicillin to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Urea Solution:

Composition per 100.0mL:

Urea............................................................................................... 6.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 65.0mL of cooled, sterile base agar,

aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se

rum, 2.0mL of sterile penicillin solution, 3.0mL of sterile MES buffer

solution, and 0.2mL of sterile urea solution. Mix thoroughly. Pour into

10mm × 35mm Petri dishes in 5.0mL volumes. Allow plates to stand

overnight at 25°C to remove excess surface moisture.

Use: For the isolation and cultivation of Ureaplasma species.

在线客服

U Agar Plates (Ureaplasma Agar Plates) (MES Agar)