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Urea Agar (Urease Test Agar) (Urea Agar Base, Christensen)

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Agar

(Urease Test Agar)

(Urea Agar Base, Christensen)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea............................................................................................. 20.0g

Agar ............................................................................................ 15.0g

NaCl .............................................................................................. 5.0g

KH2PO4......................................................................................... 2.0g

Peptone.......................................................................................... 1.0g

Glucose ......................................................................................... 1.0g

Phenol Red................................................................................ 0.012g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize. Add agar to distilled/deionized water and bring volume

to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically

add the 100.0mL of sterile basal medium. Mix thoroughly. Distribute

into sterile tubes. Allow tubes to solidify in a slanted position.

Use: For the differentiation of a variety of microorganisms, especially

members of the Enterobacteriaceae, aerobic actinomycetes, strepto

cocci, and nonfermenting Gram-negative bacteria, on the basis of ure

ase production.