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Urea Agar Base

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Agar Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

NaCl .............................................................................................. 5.0g

Na2HPO4 ....................................................................................... 1.2g

Peptone.......................................................................................... 1.0g

Glucose ......................................................................................... 1.0g

KH2PO4......................................................................................... 0.8g

Phenol Red................................................................................ 0.012g

Urea solution............................................................................50.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Urea Solution:

Composition per 100.0mL:

Urea............................................................................................. 40.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 20 min at 10 psi

pressure–115°C. Cool to 50°C. Aseptically add 50.0mL of sterile urea

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes. Allow tubes to solidify in a slanted position.

Use: For the detection of Proteus species based on rapid urease activ

ity and the identification of other members of the Enterobacteriaceae

based on urease activity. Urease-positive bacteria turn the medium

pink.