培养基配方

您现在的位置：网站首页 >> 培养基配方

Urea Semisolid Medium

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 450.0:

Solution A ..............................................................................400.0mL

Solution B ................................................................................50.0mL

Solution A:

Composition per 400.0mL:

Pancreatic digest of casein............................................................ 6.0g

Yeast extract.................................................................................. 2.0g

NaCl .............................................................................................. 1.0g

Yeast extract.................................................................................. 0.8g

Agar .............................................................................................. 0.3g

L-Cystine ....................................................................................... 0.1g

Thioglycolic acid .....................................................................0.12mL

pH 7.2 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 60°C.

Solution B:

Composition per 50.0mL:

Urea............................................................................................... 8.0g

Na2HPO4 ....................................................................................... 3.8g

KH2PO4....................................................................................... 3.64g

Yeast extract................................................................................ 0.04g

Phenol Red.................................................................................4.0mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 400.0mL of sterile

solution A and 50.0mL of sterile solution B. Mix thoroughly. Asepti

cally distribute into sterile screw-capped tubes in 7.0mL volumes. Pass

the tubes into an anaerobic chamber containing 85% N2 + 10% H2 +

5% CO2 for 60 min. Close screw caps tightly.

Use: For the cultivation and differentiation of anaerobic bacteria based on

their production of urease. Bacteria that produce urease turn the medium

bright red.