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Urea Test Broth

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Test Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea............................................................................................. 20.0g

Na2HPO4....................................................................................... 9.5g

KH2PO4......................................................................................... 9.1g

Yeast extract.................................................................................. 0.1g

Phenol Red.................................................................................. 0.01g

Urea solution..........................................................................100.0mL

Urea Solution:

Composition per 100.0mL:

Urea............................................................................................. 20.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile urea solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes in 3.0mL volumes.

Use: For the cultivation and differentiation of members of the Enter

obacteriaceae and aerobic actinomycetes based on their production of

urease. Bacteria that produce urease turn the medium bright red.