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Ureaplasma Medium (DSMZ Medium 1096)

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Ureaplasma Medium

(DSMZ Medium 1096)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1005.0mL:

Solution A ..............................................................................700.0mL

Solution B ..............................................................................305.0mL

pH 6.0 ± 0.2 at 25°C

Solution A:

Composition per 700.0mL:

Pancreatic digest of casein............................................................ 7.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Beef heart, solids from infusion.................................................... 2.0g

Urea .............................................................................................. 0.4g

L-Cysteine-HCl·2H2O ................................................................... 0.1g

DNA, fish sperm .......................................................................... 0.2g

Phenol Red ................................................................................. 0.02g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 6.0.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Solution B:

Composition per 305.0mL:

Horse serum ..........................................................................200.0mL

Yeastolate solution.................................................................100.0mL

Isovitalex....................................................................................5.0mL

Yeastolate Solution:

Composition per 100.0mL:

Yeastolate.................................................................................... 20.0g

Preparation of Yeastolate Solution: Add yeastolate to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Solution B: Combine components. Filter sterilize.

Preparation of Medium: Aseptically add 305.0mL solution B to

700.0mL solution A. Mix thoroughly.

Use: For the cultivation of Ureaplasma spp.