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Urease Test Broth (Urea Broth)

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Urease Test Broth

(Urea Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea............................................................................................. 20.0g

Na2HPO4....................................................................................... 9.5g

KH2PO4......................................................................................... 9.1g

Yeast extract.................................................................................. 0.1g

Phenol Red.................................................................................. 0.01g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep

tically distribute into sterile tubes or flasks.

Use: For the differentiation of organisms, especially the Enterobacteri

aceae, on the basis of urease production. Urease-positive bacteria turn the

medium pink.