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DSMZ培养基383b组分A基础/DSMZ383b组分A基础
Desulfonauticus Medium Base,DSMZ medium 383b Solution A Base,DSMZ Medium 383b/DSMZ Medium383b/DSMZ 383b/DSMZ383b
产品编号: MD348
产品名称: DSMZ培养基383b组分A基础/DSMZ383b组分A基础
英文名称: Desulfonauticus Medium Base
DSMZ medium 383b Solution A Base
DSMZ Medium 383b/DSMZ Medium383b/DSMZ 383b/DSMZ383b
生产厂家: 山东拓普生物工程有限公司
产品用途: 用于制备DSMZ培养基383b
浏览次数: 538
 
编号 规格 贮藏条件 保存期限 目录价 会员价 数量
MD348B250g/瓶常温3年请登录请登录请登录
配套试剂
产品详情
培养基配方(每升)
Na2SO4                    3.0g   
KH2PO4                       0.2g       
NH4Cl                        0.3g
NaCl                        21.0g
MgCl2·6H2O                  3.0g
KCl                          0.5g
CaCl2·2H2O                 0.15g
Sodium resazurin(刃天青钠)0.0005g    
使用说明
称取本品28.15g于970ml去离子水中,加入微量元素溶液SL-10(SD029)和亚硒酸盐-钨酸盐溶液(SD052)各1支,搅拌溶解,通入80%H2和20%CO2的混合气体不低于30分钟使pH低于6.0,装入厌氧容器中,121℃灭菌15分钟。
制备DSMZ培养基383b时,另制备并加入溶液B(1g碳酸钠、20ml水,121℃灭菌15分钟)、C(1支SD092)以及D(取4mlSD104),混合,使用无菌的酸碱溶液调节pH至7.0-7.2。备用

特殊说明
383b: DESULFONAUTICUS MEDIUM
Solution A
Solution B
Solution C
Solution D
972.00
20.00
1.00
13.00
ml
ml
ml
ml
1. Solution A is sparged with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at
least 30 min), then dispensed under the same gas atmosphere into anoxic Hungate-type
tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved
separately under 80% N
2 and 20% CO2 gas atmosphere. Solutions C and E are autoclaved
under 100% N
2 gas. Solution D is prepared under 100% N2 gas and filter-sterilized. To
complete the medium appropriate amounts of solutions B to E are added to the sterile
solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2.
2. After inoculation pressurize vials to 2 bar overpressure with sterile 80% H
2 and 20% CO2
gas mixture.
3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N
2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 4206, DSM 15269: Supplement medium with 1.00 g/l Na-acetate, 2.00 g/l
Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from
sterile anoxic stock solutions prepared under N
2 gas.
For DSM 15286, DSM 21156, DSM 101864: Supplement medium with 1.00 g/l Na-acetate
and 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium
from sterile and anoxic stock solutions. Vitamins are sterilized by filtration. Adjust pH of
medium to 6.5 - 6.7 prior to inoculation.
For DSM 111414: Adjust pH of final medium to 6.6.
Solution A
Na2SO4
KH2PO4
NH4Cl
NaCl
MgCl
2 x 6 H2O
KCl
CaCl
2 x 2 H2O
Trace element solution SL-10
Selenite-tungstate solution
Sodium resazurin (0.1% w/v)
Distilled water
3.00
0.20
0.30
21.00
3.00
0.50
0.15
1.00
1.00
0.50
970.00
g
g
g
g
g
g
g
ml
ml
ml
ml

Solution B
Na2CO3
Distilled water
1.00
20.00
g
ml
Solution C
Wolin's vitamin solution (10x) 1.00 ml
Solution D
Na2S x 9 H2O
Distilled water
0.40
13.00
g
ml
Selenite-tungstate solution (from medium 385)
NaOH
Na
2SeO3 x 5 H2O
Na
2WO4 x 2 H2O
Distilled water
0.50
3.00
4.00
1000.00
g
mg
mg
ml
Trace element solution SL-10 (from medium 320)
HCl (25%)
FeCl
2 x 4 H2O
ZnCl
2
MnCl2 x 4 H2O
H
3BO3
CoCl2 x 6 H2O
CuCl
2 x 2 H2O
NiCl
2 x 6 H2O
Na
2MoO4 x 2 H2O
Distilled water
10.00
1.50
70.00
100.00
6.00
190.00
2.00
24.00
36.00
990.00
ml
g
mg
mg
mg
mg
mg
mg
mg
ml
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
Biotin
Folic acid
Pyridoxine hydrochloride
Thiamine HCl
Riboflavin
Nicotinic acid
Calcium D-(+)-pantothenate
Vitamin B
12
20.00
20.00
100.00
50.00
50.00
50.00
50.00
1.00
mg
mg
mg
mg
mg
mg
mg
mg
p-Aminobenzoic acid                                         50.00                               mg
(DL)-alpha-Lipoic acid                                       50.00                               mg
Distilled water                                                  1000.00                             ml

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