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Salt Colistin Broth

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Salt Colistin Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Peptone..............................................................10.0g

Yeast extract........................................................3.0g

Colistin solution............................................ 10.0mL

pH 7.4 ± 0.2 at 25°C

Colistin Solution:

Composition per 10.0mL:

Colistin methane sulfonate.........................500,000U

Preparation of Colistin Solution: Add colistin

methane sulfonate to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept colistin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat until dissolved. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C. Aseptically add

sterile colistin solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of halophilic Vibrio species.