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Tarshis Blood Agar

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tarshis Blood Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Beef heart infusion..........................................500.0g

Agar ..................................................................15.0g

Meat peptone ....................................................10.0g

NaCl....................................................................5.0g

Penicillin G, sterile.....................................100,000U

Sheep blood, sterile..................................... 300.0mL

Glycerol ........................................................ 10.0mL

pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept sheep blood and penicillin G, to distilled/deion

ized water and bring volume to 750.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile sheep blood and

sterile penicillin G. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Mycobacte

rium tuberculosis.