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Trypticase Soy Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................28.0g

NaCl..................................................................10.0g

Pancreatic digest of casein..................................2.5g

Yeast extract........................................................2.5g

Base medium .............................................. 100.0mL

Phosphate buffer ......................................... 100.0mL

pH 7.2 ± 0.2 at 25°C

Base Medium:

Composition per liter:

MgCl2·6H2O.......................................................2.0g

Titriplex I ............................................................1.0g

CaSO4·2H2O.......................................................0.4g

NaOH-pellets ......................................................0.2g

Fe-citrate solution ........................................... 5.0mL

Trace elements solution .................................. 5.0mL

Fe-Citrate Solution:

Composition per 100.0mL:

Fe-citrate...........................................................0.25g

Preparation of Fe-Citrate Solution: Add Fe

citrate to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per 5.0mL:

CuCl2·2H2O...................................................50.0mg

Titriplex I .......................................................12.8mg

FeCl2·4H2O......................................................1.0mg

MnCl2·4H2O....................................................0.5mg

CoCl2·6H2O.....................................................0.3mg

ZnCl2 ...............................................................0.2mg

Na2MoO4·2H2O..............................................50.0ng

NiCl2·6H2O.....................................................50.0ng

H3BO3.............................................................20.0ng

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 5.0mL. Mix thoroughly.

Preparation of Base Medium: Add components

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly.

Phosphate Buffer:

Composition per liter:

Na2HPO4·12H2O ..............................................43.0g

KH2PO4 ............................................................5.44g

Preparation of Phosphate Buffer: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, ex

cept phosphate buffer, and bring volume to 900.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Aseptically add 100.0mL of sterile

phosphate buffer. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Lacto

coccus lactis and Lactococcus plantarum.

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Trypticase Soy Yeast Extract Agar