Caminicella Medium (DSMZ Medium 964)

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caminicella Medium
(DSMZ Medium 964)

培养基配方
Composition per liter:
Sea salt.....................................................30.0g
Sulfur, powdered.............................................12.0g
PIPES buffer.................................................6.05g
D (+)-glucose.................................................5.0g
Peptone.......................................................1.0g
Yeast extract.................................................0.5g
Resazurin ...................................................0.5mg
Na2S·9H2O solution.........................................10.0mL
pH 7.5 ± 0.2 at 25°C
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O...................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to
25°C while sparging with 80% N2 + 20% CO2 . Adjust pH to 7.2–7.4.
Distribute to anaerobe tubes or bottles under 80% N2 + 20% CO2 . Au-
toclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobi-
cally add per liter of medium, 10.0mL sterile Na2S·9H2O solution. Mix
thoroughly. The final pH should be 7.5.
Use:  For the cultivation of Caminicella sporogenes.