Camphor Minimal Medium

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Camphor Minimal Medium

培养基配方
Composition per liter:
Agar ...........................................................20.0g
K2HPO4 ..........................................................4.4g
NH4Cl ...........................................................2.1g
KH2PO4 ..........................................................1.7g
100× salt solution............................................10.0mL
100X Salt Solution:
Composition per liter:
MgSO4 ..........................................................19.5g
FeSO4 ·7H2O.....................................................5.0g
MnSO4 ·H2O......................................................5.0g
Ascorbic acid ...................................................1.0g
CaCl2 ·2H2O.....................................................0.3g
Preparation of 100X Salt Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes. Allow to cool to room temperature. Invert Petri dishes. Spread
0.2mL of 2M D- (+) camphor solution in methylene chloride (CH2Cl2 )
on the inside cover of each plate.
Use:  For the cultivation and maintenance of Pseudomonas putida.