Campy THIO Medium

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campy THIO Medium

培养基配方
Composition per liter:
Pancreatic digest of casein........................................20.0g
Agar...............................................................15.0g
NaCl................................................................2.5g
K2HPO4 .............................................................1.5g
Sodium thioglycolate................................................0.6g
L- Cystine..........................................................0.4g
Na2SO3 .............................................................0.2g
Antibiotic supplement.............................................10.0mL
Antibiotic Supplement:
Composition per 10.0mL:
Cephalothin.......................................................15.0mg
Vancomycin........................................................10.0mg
Trimethoprim.......................................................5.0mg
Amphotericin B ....................................................2.0mg
Polymyxin B........................................................2500U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile antibiotic solution. Mix thoroughly. Aseptically distribute into
sterile screw-capped tubes in 3.0mL volumes for 1.5cm swabs or
5.0mL volumes for 3.0cm swabs.
Use: For the maintenance—as a holding or transport medium—of
Campylobacter species isolated from clinical specimens on swabs.