Campylo Thioglycollate HiVeg Medium Base with Selective Supplement

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylo Thioglycollate HiVeg Medium Base
with Selective Supplement

培养基配方
Composition per liter:
Plant hydrolysate ..............................................20.0g
NaCl.............................................................2.5g
Agar.............................................................1.6g
K2HPO4 ..........................................................1.5g
Na-thioglycollate................................................0.6g
L -Cystine.......................................................0.4g
Na2SO3 ..........................................................0.2g
Selective supplement...........................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without selective supplement, is available as a
premixed powder from HiMedia.
Selective Supplement:
Composition per 10.0mL:
Cephalothin...................................................15.0mg
Vancomycin....................................................10.0mg
Trimethoprim...................................................5.0mg
Amphotericin B ................................................2.0mg
Polymyxin B sulfate ...........................................2500U
Preparation of Selective Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except selective sup-
plement and sodium deoxycholate solution, to distilled/deionized wa-
ter and bring volume to 990.0mL. Mix thoroughly. Heat with frequent
agitation and boil for 1 min to completely dissolve. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C. Add 10.0mL of ster-
ile selective supplement. Mix thoroughly.
Use: For the cultivation of Campylobacter spp.