CHI 1776 Medium

[所属分类:培养基配方] [发布时间:2020-4-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CHI 1776 Medium

培养基配方
Composition per liter:
Pancreatic digest of casein..................................25.0g
Yeast extract.................................................7.5g
Glucose solution ...........................................25.0mL
Tris·HCl buffer, 1M, pH 7.5................................20.0mL
Diaminopimelic acid solution................................10.0mL

Thymidine solution..........................................10.0mL
MgCl2  solution..............................................5.0mL
Glucose Solution:
Composition per 100.0mL:
D- Glucose...................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Diaminopimelic Acid Solution:
Composition per 100.0mL:
meso-Diaminopimelic acid.....................................1.0g
Preparation of Diaminopimelic Acid Solution: Add meso-di-
aminopimelic acid to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Thymidine Solution:
Composition per 100.0mL:
Thymidine......................................................0.4g
Preparation of Thymidine Solution: Add thymidine to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
MgCl2 Solution:
Composition per 100.0mL:
MgCl2 ........................................................9.52g
Preparation of MgCl2 Solution: Add MgCl2 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion, diaminopimelic acid solution, thymidine solution, and MgCl2  so-
lution, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 25.0mL of sterile glucose solution, 10.0mL of sterile diamin-
opimelic acid solution, 10.0mL of sterile thymidine solution, and
5.0mL of sterile MgCl2  solution, Mix thoroughly. Distribute into sterile
tubes or flasks.
Use:  For the cultivation of Escherichia coli.