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Tissue Culture Earle Solution (TC Earle Solution)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tissue Culture Earle Solution

(TC Earle Solution)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

NaCl .............................................................................................. 6.8g

NaHCO3 ........................................................................................ 2.2g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2·2H2O................................................................................... 0.2g

NaH2PO4 ................................................................................... 0.125g

MgSO4·7H2O ................................................................................ 0.1g

Phenol Red (1% solution) ..........................................................2.0mL

pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Earle Solution: Add compo

nents, except Phenol Red, to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Add 2.0mL of Phenol Red solution.

Adjust pH to 7.2–7.4. Filter sterilize.

Use: For use in tissue culture and virus preparations.